Journal: The Journal of allergy and clinical immunology
Article Title: Periostin is required for maximal airways inflammation and hyperresponsiveness in mice
doi: 10.1016/j.jaci.2014.05.029
Figure Lengend Snippet: DCs from Postn (+/+) mice are sufficient for allergic airway inflammation. A, B. Lungs from HDM-exposed F6 B6;129 Postn (+/+) mice (A) and Postn (−/−) littermates (B) were immunostained for periostin (green) and I-A/I-E (red), a mouse DC marker (original magnification 100X, inset 800X). C-F. HDM-matured differentiated bone marrow-derived DCs from Postn (+/+) mice or Postn (−/−) mice were transferred to Postn (−/−) mice intratracheally and challenged with PBS or HDM. PAS-stained lung sections from PBS- (C, E) and HDM-treated mice (D, F) treated with Postn (+/+) DCs (C, D) or Postn (−/−) DCs (E, F). (100x magnification, bar is 100 μm.) These figures are representative of three separate experiments.
Article Snippet: For immunohistochemistry, sections were probed with rabbit anti-periostin and stained using a biotinylated anti-rabbit IgG-avidin horseradish peroxidase and diaminobenzidine detection system (Vector Labs, Burlingame, CA).
Techniques: Marker, Derivative Assay, Staining